Daniel Stein

Marbach-Ad, G., Briken, V., Frauwirth, K., Gao, L., Hutcheson, S.W., Joseph, S.W., Mosser, D., Parent, B, Shields, P., Song, W., Stein, D.C., Swanson, K., Thompson, K.V., Yuan, R., Smith, A.C. 2007. A Faculty Team Works to Create Content Linkages among Various Courses to Increase Meaningful Learning of Targeted Concepts of Microbiology. CBE Life Sciences Education . 6: 155-162

Gili Marbach-Ad, Volker Briken, Najib El-Sayed, Kennneth Frauwirth, Brenda Fredericksen, Steven Hutcheson, Lian-Yong Gao, Sam Joseph, Vincent Lee, Kevin S. McIver, David Mosser, B. Booth Quimby, Patricia Shields, Wenxia Song, Daniel C. Stein, Robert T. Yuan and Ann C. Smith 2009."Assessing Student Understanding of Host Pathogen Interactions Using a Concept Inventory" J Microbiol Biol Ed. 10.

Dr. Stein's research focuses on the mechanisms by which the human pathogen, Neisseria gonorrhoeae synthesizes and regulates the expression of its outer membrane antigens, and how changes in the expression of these components effect disease outcome. Students use molecular genetic, cellular, immunological and biochemical techniques to further our understanding of gonococcal disease.

Ram, S., A.D. Cox, J. C. Wright, U. Vogel, S. Getzlaff, R. Boden, J. S. Plested, S. Meri, S. Gulati, D. C. Stein, J. C. Richards, E. R. Moxon and P. A. Rice. 2003. Neisserial lipooligosaccharide is a target for complement C4b: inner core phosphoethanolamine residues define C4b linkage specificity. J. Biol. Chem. 278:50853-62.

Braun, D. C and D. C. Stein. 2004. The lgtABCDE gene cluster, involved in LOS biosynthesis in Neisseria gonorrhoeae, contains multiple promoter sequences. J. Bacteriol. 186:1038-1049.

O'Connor, E. T., A. Piekarowicz, K. V. Swanson, J. M. Griffiss, and D. C. Stein. 2006. Biochemical analysis of Lpt-3, a protein responsible for phosphoethanolamine addition to lipooligosaccharide of pathogenic Neisseria. J. Bacteriol.188:1039-1048.

Patrone,J. S. Bish and D. C. Stein. 2006. TNF-independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response. J. Immunol. 177:1314-1322.

Patrone, J.B. and D. C. Stein. 2007. Sialylation of Gonococcal Lipooligosaccharide Alters Human Monocytic Cytokine Profile. BMC Microbiology 7:7

Piekarowicz, A., A. Klyz, M. Adamczyk-Poplawska, M. Majchrzak and D. C. Stein. 2007. Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae. BMC Microbiology 5:66.

O'Connor, E. T., K. V. Swanson, K. L. Fluss, J. M. Griffiss, and D. C. Stein. 2008. Structural Requirements for Monoclonal Antibody 2-1-L8 Recognition on Neisserial Lipooligosaccharides. Hybridoma 27:71-79.

Bish, S. E., W. Song, and D.C. Stein. 2008. Quantification of bacterial invasion into host cells using a beta-lactamase reporter strain: Neisseria gonorrhoeae invasion into cervical epithelial cells requires bacterial viability. Microbes Infect. 10:1182-1191

O'Connor, E. T., H. Zhou, K. Bullock, K. V. Swanson, J. M. Griffiss, V. N. Reinhold, C. J. Miller and D. C. Stein. 2009. Structural characterization of the lipopolysaccharide O-antigen repeat made by Neisseria sicca. J. Bacteriol. 191:3311-3320

N.I.H. Training Grant: Predoctoral Fellowship, 1977-81
Miles Laboratory: Postdoctoral Training Fellowship, 1981-83
Maryland General Research Board, Semester off to do research, 1990
University of Maryland, Inventor of the Year, 1994
Career Development Award from NIH (1997-2000)
University of Maryland Rainmaker Award (2003)
University of Maryland, Inventor of the Year, runnerup (2005)
Honorary Member of the Cuban Society of Immunology

MS. University of Rochester, 1981

Ph.D. University of Rochester, 1981